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lncrna + mrna human gene expression microarray v4.0 platform  (Agilent technologies)


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    Agilent technologies lncrna + mrna human gene expression microarray v4.0 platform
    Lncrna + Mrna Human Gene Expression Microarray V4.0 Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lncrna + mrna human gene expression microarray v4.0 platform/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    lncrna + mrna human gene expression microarray v4.0 platform - by Bioz Stars, 2026-04
    90/100 stars

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    LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated <t>lncRNAs</t> identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.
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    LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated lncRNAs identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.

    Journal: Frontiers in Oncology

    Article Title: Transforming Growth Factor-Beta-Regulated LncRNA-MUF Promotes Invasion by Modulating the miR-34a Snail1 Axis in Glioblastoma Multiforme

    doi: 10.3389/fonc.2021.788755

    Figure Lengend Snippet: LncRNA-MUF is overexpressed in GBM, and it is induced by TGF-β. (A) Heatmap representing the relative abundance of differentially expressed genes (DEGs, ≥1.5-fold, p < 0.05) in T98G cells upon TGF-β1 (10 ng/ml) treatment for 24 h. (B) Pie chart representing the class of DEGs (p < 0.05) identified from genome-wide microarray screening in T98G GBM cells upon TGF-β1 treatment for 24 h. (C) Validation of top TGF-β-regulated lncRNAs identified from microarray screening using qRT-PCR in T98G cells. RNA samples were analyzed by qRT-PCR, and error bars represent the mean ± SEM from three independent experiments. *Significant change in TGF-β-treated cells compared to control cells (p < 0.05). (D) LncRNA-MUF expression analysis in glioma tissues from the CGGA database. Elevated expression of MUF in GBM IV compared to lower-grade gliomas (p=1.6e- 17 ). (E) LncRNA-MUF levels are elevated in IDH wild-type GBM patients compared to IDH mutant cases as analyzed from the CGGA database (ANOVA, p = 9.7e- 28 ). (F) Kaplan–Meier survival analysis of lncRNA-MUF expression in glioma samples from CGGA databases shows that a high lncRNA-MUF expression is associated with poor overall survival in primary and recurrent GBM patients (p = 0.0063). Red line represents high lncRNA-MUF expression group, and green line represents the low lncRNA-MUF expression group.

    Article Snippet: Agilent SurePrint G3 Gene Expression Microarrays for Human (v3) for lncRNAs, containing 30,606 lncRNAs and 37,756 RefSeq-coding transcripts, were used to interrogate lncRNA and mRNA changes in vehicle versus TGF-β1 (10 ng/ml)-treated T98G cells after 24 h. RNA was isolated using MN NucleoSpin RNA Plus isolation kit (Cat. No. 740984.5).

    Techniques: Genome Wide, Microarray, Quantitative RT-PCR, Expressing, Mutagenesis